Identification and Imaging of 15 N labeled cells with ToF-SIMS
نویسندگان
چکیده
منابع مشابه
C60-ToF SIMS imaging of frozen hydrated HeLa cells.
Sample preparation continues to be a major challenge for secondary ion mass spectrometry studies of biological materials. Maintaining the native hydrated state of the material is important for preserving both chemical and spatial information. Here, we discuss a method which combines a sample wash and dry protocol discussed by Berman et al1 (1) followed by plunge freezing in liquid ethane for a ...
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Time-of-flight secondary ion mass spectrometry (TOF-SIMS) was introduced into cosmochemistry about a decade ago. Major advantages of TOF-SIMS compared to other ion microprobe techniques are (a) parallel detection of all secondary ions with one polarity in a single measurement – both polarities in subsequent analyses, (b) high lateral resolution, (c) sufficient mass resolution for separation of ...
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1 Vienna University of Technology, Institute of Chemical Engineering Laboratory for Chemometrics, Getreidemarkt 9/166, A-1060 Vienna, Austria [email protected], www.lcm.tuwien.ac.at 2 Vienna University of Technology, Institute of Statistics and Probability Theory Wiedner Hauptstrasse 8-10, A-1040 Vienna, Austria [email protected], www.statistik.tuwien.ac.at/public/filz 3 Max Pl...
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A novel, trehalose-glycerol matrix was utilized to generate high-resolution, TOF-SIMS images of macrophages and glial cells. Viable cells incubated in 50 mM trehalose, then lyophilized in a 50 mM trehalose, 10-15% (w/w) glycerol rinse, are preserved and chemically profiled. These experiments demonstrate the utility of the disaccharide matrix as an efficient, cost-effective alternative to cryoge...
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ژورنال
عنوان ژورنال: Surface and Interface Analysis
سال: 2010
ISSN: 0142-2421
DOI: 10.1002/sia.3679